We offer the possibility to test more than 500 crystallisation conditions, thanks to the use of a nano-drop dispenser robot (Honeybee) with a wide choice of commercial screening kits (primarily the Nextal series by Quiagen). These crystallisation tests can be performed in the presence of ligands or additives (for example detergents). The technique used for these assays is the sitting drop method, which requires at least 0.5 microL of protein solution per 96-well plate. If these initial tests result in (micro-) crystals, screening on the crystallogenesis conditions can be performed in order to improve the quality and size of the crystals.
The platform for crystallography is located on site of the Station Biologique de Roscoff. It is composed of two parts: i) Crystallogenesis ii) Diffraction - 3D Structure Determination & Refinement.
The personnel of the platform accompanies and guides any structural project that uses the approach of protein crystallography. More specifically the personnel can :
Assure and accompany the access to the machines (crystallization robot & diffractometer, as well as access to synchrotron radiation)
Evaluate the feasibility (time needed, cost, novelty, difficulty..) of presented projects
Follow and accompany the projects along all steps of the approach
> A unit for production and detection of X-rays : rotating anode NONIUS/BRUKER.
Diffractometer with microfocus, equipped with a four-circle goniometer and a bi-dimensional CCD detector.
Cryocooling facility and installation to freeze, store and collect the data at 100 K
> Nano-drop dispensing robot for crystallogenesis
A nano-drop dispensing robot of the type ‘Honeybee’ from Protein Solutions, for the setup of crystallization trials in 96-well format.
Rooms/storage to setup trials at 18°C and 4°C.
> Access to a informatics server and dedicated software
1 calculation cluster with 6 processors (Linux); network of 6 PC (linux) with graphical devices for data treatment and analysis. Support for the use of all classical software suites used in protein crystallography for interactive construction, visualization and analysis of 3D biomolecule structures.